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Table 2 Physiological and microarray quality parameters for aerobic, glucose-limited chemostat cultures grown at a dilution rate of 0.03 h-1

From: Exploring and dissecting genome-wide gene expression responses of Penicillium chrysogenum to phenylacetic acid consumption and penicillinG production

  DS17690 - PAA DS17690 + PAA DS50661 - PAA DS50661 + PAA
Y sx a (g·g -1 ) 0.37 ± 0.01 0.35 ± 0.01 0.39 ± 0.01 0.36 ± 0.01
q pen b (μmol·g -1 ·h -1 ) 0.00 ± 0.00 19.81 ± 1.47 0.00 ± 0.00 0.00 ± 0.00
q PAA c (μmol·g -1 ·h -1 ) 0.00 ± 0.00 24.04 ± 2.38 0.00 ± 0.00 5.60 ± 0.38
q CO2 d (mmol·g -1 ·h -1 ) 1.15 ± 0.08 1.42 ± 0.11 1.16 ± 0.05 1.44 ± 0.10
q O2 e (mmol·g -1 ·h -1 ) 1.19 ± 0.11 1.42 ± 0.17 1.16 ± 0.11 1.43 ± 0.08
Avg CV f 0.21 0.18 0.17 0.14
Pc actA g 4190 ± 170 3560 ± 360 3450 ± 390 3030 ± 680
Pc gdh2 h 1240 ± 120 1140 ± 270 1020 ± 170 1060 ± 180
Repeats (n) 3 4 3 3
  1. P. chrysogenum strains DS17690 (penicillinG high producing) and DS50661 (lacking a functional penicillin gene cluster) were grown in the presence and absence of phenylacetic acid (PAA) in independent glucose-chemostat cultures at D = 0.03 h-1. Results are the averages ± S.D. (σn-1).
  2. a biomass yield on glucose (g of biomass per g of glucose consumed)
  3. b biomass specific penicillinG production rate. In addition to penicillinG, intermediates and byproducts are formed, which accounts for ~6% of the consumed PAA [30] (μmol of penicillin G produced per g of dry weight biomass and per · hour)
  4. c Biomass specific phenylacetate consumption rate (μmol of phenylacetate consumed per g of dry weight biomass and per hour)
  5. d Biomass specific CO2 production rate (mmol of CO2 produced per g of dry weight biomass and per hour)
  6. e Biomass specific O2 consumption rate (mmol of O2 consumed per g of dry weight biomass and per hour)
  7. f the average coefficient of variation (standard deviation divided by the mean) for all genes except the genes with mean below 12
  8. g encoding actin; average hybridization signal and standard deviation
  9. h encoding glutamate dehydrogenase (NAD+ dependent); average hybridization signal and standard deviation