Table 4 Primers used to amplify fragments of Crassostrea hongkongensis mitochondrial genome.

Order	Primer name	Sequence (5'-3')	Length	position	Product size (bp)
1*	HK-4343F	TTAGAGTTCCGTTTCACCCG	20	4343	2470, 4617
	HK-6812R	CTTTCGCTTCAATTTAGTTAGT	22	6812, 8959
2*	HK-6569F	GGTTCTGGTATAATGTTAGCT	21	6569, 8716	824, 2971
	HK-7392R	ATTACTCTCTTTTTACTCCC	20	7392, 9539
3*	HK-9412F	CTAGGTCAGGTCGAAGTGCT	20	7265, 9412	1016, 3163
	HK-10427R	AGAGCACAGGTGTTGGGTGA	20	10427
4	HK-5807F	GTCTCATAATCCGAAAGTGGTT	22	5807	2658
	HK-8464R	CTTATACTTGGGCTACTTTCTT	22	8464
5	HK-8138F	GGTGCTCACTAAATCAGTATGT	22	8138	1905
	HK-10043R	ATGAAGATAGTGACGGAAACCC	22	10043

*Primer pairs from Yu et al. (2008). Because one or both of the primers are located in the duplicated rrnS gene, two fragments are expected but only the shorter one is actually amplified.

ISSN: 1471-2164