Table 4 Results of the white-throated sparrow "morph" assay using the Dra I restriction site located in the VIP gene [50]. Species heterozygous for the Dra I restriction site show 3 fragments (F1-F3); species homozygous for the Dra I restriction site show 2 bands, one smaller band at approximately 72–89 bp and one larger band at 185–190 bp (F1 and F2); finally, homozygotes that do not carry the Dra I restriction site show only a single band at 284 bp (F3).

White-throated sparrow

(Zonotrichia albicollis)

2^m/2^m

Homozygous – Dra I site

88

189

-

0

0

2^m/2 (White Morph)

Heterozygous

88

189

284

278

50.9

2/2 (Tan Morph)

Homozygous – No site

-

-

284

268

49.1

White-crowned sparrow

(Z. leucophyrs)

Homozygous – Dra I site

80

190

-

2

16.7

Heterozygous

80

190

284

4

33.3

Homozygous – No site

-

-

284

6

50.0

Golden-crowned sparrow

(Z. atricapilla)

Homozygous – Dra I site

83

190

-

0

0

Heterozygous

83

190

284

3

100

Homozygous – No site

-

-

284

0

0

Harris's sparrow

(Z. querula)

Homozygous – Dra I site

72/73

88/91

-

1

25.0

Heterozygous

72 or 73

88 or 91

284

0

0

Homozygous – No site

-

-

284

3

75.0

Rufous-collared sparrow

(Z. capensis)

Homozygous – Dra I site

89

191

-

10

100

Dark-eyed junco

(Junco hyemalis)

Slate-colored

hyemalis

Homozygous – Dra I site

89

189

-

4

100

Oregon

oreganus

Homozygous – Dra I site

89

189

-

3

100

Gray-headed

caniceps

Homozygous – Dra I site

89

189

-

1

100

Song sparrow

(Melospiza melodia)

Homozygous – Dra I site

89

185

-

5

100

Swamp sparrow

(M. georgiana)

Homozygous – Dra I site

89

186/189

-

5

100

Purple finch

(Carpodacus purpureus)

Homozygous – Dra I site

89

193

-

1

100