RExPrimer: an integrated primer designing tool increases PCR effectiveness by avoiding 3' SNP-in-primer and mis-priming from structural variation

Table 1 The designed primers for CYP2D6 analysis obtained from RExPrimer

Name	Sequence 5' → 3'	Purpose
CYP2D6_1F	GGCCTACCCTGGGTAAGGGCCTGGAGCAGGA	whole gene amplification
CYP2D6_1R	CTCAGCCTCAACGTACCCCTGTCTCAAATGCG
CYP2D6_2F	GAGACTCCTCGGTCTCTCG	penta-plex PCR
CYP2D6_2R	TAATGCCTTCATGGCCACGCG
CYP2D6_3F	AGGCCTTCCTGGCAGAGATGAAG	penta-plex PCR
CYP2D6_3R	CCCCTGCACTGTTTCCCAGA
CYP2D6_4F	CCAGCCACCATGGTGTCTTTG	penta-plex PCR
CYP2D6_4R	GCCTCAACGTACCCCTGTCTC
DMD_F	TTGTCGGTCTCCTGCTGGTCAGTG	one-copy internal standard in penta-plex PCR
DMD_R	CAAAGCCCTCACTCAAACATGAAGC
LDL_F	TACAAGTGCCAGTGTGAGGAAG	two-copy internal standard in penta-plex PCR
LDL_R	GTGCAAAGTTCAGAGGATGAAACT

ISSN: 1471-2164