Figure 4
From: Adaptation of Hansenula polymorpha to methanol: a transcriptome analysis
Ultrastructural analysis of the adaptation of cells to methanol. Glucose-grown H. polymorpha WT cells were extensively analysed at different time-points after the shift to methanol by electron microscopy of KMnO4-fixed samples. (A) Control glucose-grown cell and (B) after 2 h of incubation in the presence of methanol. A clear increase in peroxisome size was observed, cross-sections of representative cells are depicted. Note the association of the organelles with strands of ER (indicated by arrow). (C) 2 hours after the shift a clear increase was observed in large vacuolar autophagic bodies, indicative of induction of autophagy. N - Nucleus, M - Mitochondrion, V - Vacuole, AV - Autophagosome. The bar represents 0.5 ?m.