Genomics based analysis of interactions between developing B-lymphocytes and stromal cells reveal complex interactions and two-way communication

Table 8 Analysis of communication pathways for mature B-cells (B-cells)

Gene expressed in MatureB	Mean Intensity	Corresponding gene in stroma	Mean Intensity	NIH3T3	Osteoblasts
chemokine (C-X-C motif) receptor 4	1275	chemokine (C-X-C motif) ligand 12	12222	23	798
epidermal growth factor receptor	145	Epiregulin	422	288	153
interleukin 2 receptor, gamma chain	2746	interleukin 7	189	92	255
Integrin alpha 4	1640	junction adhesion molecule 2	201	16	26
integrin beta 1 (fibronectin receptor beta)	235	junction adhesion molecule 2	201	16	26
integrin beta 2	1781	junction adhesion molecule 3	152	42	204
lymphotoxin A	540	lymphotoxin B receptor	965	285	519
transforming growth factor, beta 1	718	Similar to Ornithine decarboxylase (ODC)	197	146	14
epidermal growth factor receptor	145	transforming growth factor alpha	261	13	21
transforming growth factor, beta 1	718	transforming growth factor, beta receptor II	171	85	253
transforming growth factor, beta 1	718	transforming growth factor, beta receptor III	3653	3429	1152
lymphotoxin A	540	tumor necrosis factor receptor superfamily, member 1a	1974	959	1143
lymphotoxin A	540	tumor necrosis factor receptor superfamily, member 1b	108	721	108
Integrin alpha 4	1640	vascular cell adhesion molecule 1	692	182	929
integrin beta 1 (fibronectin receptor beta)	235	vascular cell adhesion molecule 1	692	182	929
integrin beta 7	942	vascular cell adhesion molecule 1	692	182	929

Tables 1, 2, 3, 4, 5, 6, 7 and 8. Receptor ligand pairs identified as possible means of communication between a model stroma cell line OP9 and sorted hematopoietic cells from the mouse bone marrow. Expression values observed in fibroblastic NIH3T3 and osteoblastic MC3T3 cells are included to allow for the identification of stromal cell specific interactions. The diagram only display receptor ligand pairs were genes on the hematopoietic array as well as of the stromal cells arrays have a normalized expression value above 100. Full lists of potential interactions can be found in Additional files 2, 3, 4, 5, 6, 7, 8 and 9. For sorted cells as well as OP9 the value is a mean out of 2 experiments for complete list of genes for each cell type see Additional files 2, 3, 4, 5, 6, 7, 8 and 9.

ISSN: 1471-2164