Figure 5
From: Increased incidence of rare codon clusters at 5' and 3' gene termini: implications for function
The population of ORFs with 5' rare codon clusters does not show a dependence on the stability of mRNA folding near the 5' terminus. The energetics of mRNA folding for nucleotides 1-40 were calculated for every ORF in E. coli and the population was separated based on the presence (gray circles, left Y-axis) or absence (black squares, right Y-axis) of a rare codon cluster in the first 13 windows, the windows that would overlap with nucleotides 1-40. The ORFs with or without 5' rare codon clusters have a similar median (ΔGfolding: -5.6 kcal/mol and -5.9 kcal/mol, respectively) and the same population distribution as seen by the linear regression when comparing the population with rare codons and those without for each ΔGfolding value (inset).