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Table 3 Bacterial strains and plasmids used in this study.

From: The Zur regulon of Corynebacterium glutamicum ATCC 13032

Strain or plasmid Relevant characteristics Source or reference
C. glutamicum ATCC 13032 wild-type strain ATCC
C. glutamicum JS2502 ATCC 13032 with defined deletion in zur This study
E. coli DH5αMCR E. coli strain used for standard cloning procedures [73]
E. coli TOP10 E. coli strain used for cloning of RACE-PCR products Invitrogen
pCR2.1-TOPO lacZα, Apr; E. coli cloning vector Invitrogen
pK18mobsacB sacB, Kmr; E. coli vector for allelic exchange [74]
pK18mobsacBzur sacB, Kmr; pK18mobsacB carrying a modified zur gene with internal deletion This study
pASK-IBA5+ PTet, strep-tag, Apr; E. coli expression vector IBA Tagnologies
pASK-IBA5+_cg2502 pASK-IBA5+ carrying the C. glutamicum zur gene This study
pEPR1 gfpuv PL , Kmr; promoter-probe vector [28]
pEPR1_prom_cg2500 gfpuvPL, Kmr; pEPR1 carrying the znr upstream region This study
pEPR1_prom_cg2502 gfpuvPL, Kmr; pEPR1 carrying the zur upstream region This study
pEPR1_prom_cg0042 gfpuvPL, Kmr; pEPR1 carrying the cg0042 upstream region This study
pEPR1_prom_cg2911 gfpuvPL, Kmr; pEPR1 carrying the cg2911 upstream region This study
pEC-XK99E P trc , lacI, Kmr; C. glutamicum expression vector [75]
pEC-XK99E_zur P trc , lacI, Kmr; pEC-XK99E vector carrying the zur gene for complementation This study