Table 3 Bacterial strains and plasmids used in this study.
From: The Zur regulon of Corynebacterium glutamicum ATCC 13032
Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
C. glutamicum ATCC 13032 | wild-type strain | ATCC |
C. glutamicum JS2502 | ATCC 13032 with defined deletion in zur | This study |
E. coli DH5αMCR | E. coli strain used for standard cloning procedures | [73] |
E. coli TOP10 | E. coli strain used for cloning of RACE-PCR products | Invitrogen |
pCR2.1-TOPO | lacZα, Apr; E. coli cloning vector | Invitrogen |
pK18mobsacB | sacB, Kmr; E. coli vector for allelic exchange | [74] |
pK18mobsacB _Δzur | sacB, Kmr; pK18mobsacB carrying a modified zur gene with internal deletion | This study |
pASK-IBA5+ | PTet, strep-tag, Apr; E. coli expression vector | IBA Tagnologies |
pASK-IBA5+_cg2502 | pASK-IBA5+ carrying the C. glutamicum zur gene | This study |
pEPR1 | gfpuv PL , Kmr; promoter-probe vector | [28] |
pEPR1_prom_cg2500 | gfpuvPL, Kmr; pEPR1 carrying the znr upstream region | This study |
pEPR1_prom_cg2502 | gfpuvPL, Kmr; pEPR1 carrying the zur upstream region | This study |
pEPR1_prom_cg0042 | gfpuvPL, Kmr; pEPR1 carrying the cg0042 upstream region | This study |
pEPR1_prom_cg2911 | gfpuvPL, Kmr; pEPR1 carrying the cg2911 upstream region | This study |
pEC-XK99E | P trc , lacI, Kmr; C. glutamicum expression vector | [75] |
pEC-XK99E_zur | P trc , lacI, Kmr; pEC-XK99E vector carrying the zur gene for complementation | This study |