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Table 2 Validation of microarray results by quantitative real-time RT-PCR (qPCR)

From: Escherichia coli infection induces distinct local and systemic transcriptome responses in the mammary gland

  

Mean fold change qPCR

Mean fold change Affymetrix

Gene

SOTA Cluster

i24h

Øn24h

n24h_u

n24h_s

i24h

n24h

SF3A1

-

-

-

-

-

-

-

HP

1

776

3.7*

3.5

4.3

207*

3.6

SAA3

1

1826*

5.3*

5.3

5.7

51.6*

5.4*

PTX3

1

32.7*

1.2

1.4

1.0

19.0*

1.2

S100A8

2

1351*

11.3*

13.9

9.2

165*

11.2*

MT2A

2

921*

63.7*

73.9

51.0

26.0*

58.8*

LBP

2

27.9*

4.0*

3.7

4.6

19.3*

3.8*

AQP3

3

-7.5*

-3.5*

-3.5

-3.5

-3.3*

-3.4*

ALOX15

3

-48.4*

-11.8*

-11.8

-11.8

-26.2*

-12.9*

ACAS2

4

-7.4*

1.2*

1.2

1.2

-2.3*

1.3*

ADHFE1

4

-2.6*

1.3

1.2

1.3

-2.1*

1.3*

PIR

5

-1.1

4.4*

5.3

3.2

-1.1

4.4*

THRSP

5

-3.3

3.8*

3.0

5.1

-1.8

3.6*

HPGD

6

-32.0*

-3.7

-3.5

-4.0

-12.3*

-3.7

LPL

6

-116*

-3.6*

-3.8

-3.2

-23.6*

-3.7

  1. *: p Value < 0.01. Detailed data: see Additional File 3
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