Figure 1From: Transcriptional and pathway analysis in the hypothalamus of newly hatched chicks during fasting and delayed feedingRelative levels of mRNA for selected genes upregulated by fasting as determined by microarray and qRT-PCR. Data were normalized to the housekeeping gene phosphoglycerate kinase 1 (PGK1), and are expressed relative to mRNA levels at hatch, which are set to the value of 1. Gene expression higher than 1 indicates upregulation compared to hatch, while gene expression levels less than 1 indicate downregulation compared to hatch. Chicks were either fully fed (FF), not fed (NF) or delayed fed (DF). Treatment groups are designated as follows: hatch (H), fed for 24 h (24FF), fasted for 24 h (24NF), fed for 48 h (48FF), fasted for 48 h (48NF), fasted for 48 h then fed for 4 h (4DF), fed for 72 h (72FF), fasted for 48 h then fed for 24 h (24DF), fed for 96 h (96FF), fasted for 48 h then fed for 48 h (48DF). Genes evaluated were: neuropeptide Y receptor 5 (NPY5R), aromatase (CYP19A), deiodinase II (DIO2), FK506 binding protein 51 (FKBP51), somatostatin receptor 5 (SSTR5), and coagulation factor C (COCH). Black bars are microarray results, and white bars are qRT-PCR results. Values within a single method with differing letters are significantly different (P < 0.05).Back to article page