Improvement of tissue preparation for laser capture microdissection: application for cell type-specific miRNA expression profiling in colorectal tumors

Table 1 Experimental conditions for tissue preparations

Experiment	Section	Ethanol fixation	Storage at -80°C	Inhibitor*	Staining	LCM	RIN & SD
Ethanol fixation (n = 48)
A-control	6	no	no	no	no	no	2.7 ± 0.5
A	6	10 min	no	no	no	no	4.8 ± 1.6
B-control	6	no	2 h	no	no	no	3.4 ± 0.8
B	6	10 min	2 h	no	no	no	6.1 ± 1.9
C-control	6	no	5 h	no	no	no	3.1 ± 1.0
C	6	10 min	5 h	no	no	no	4.9 ± 2.1
D-control	6	no	24 h	no	no	no	2.9 ± 0.7
D	6	10 min	24 h	no	no	no	4.4 ± 1.6
Rnase inhibitor (n = 12)
E-control	6	no	no	no	no	no	3.4 ± 1.9
E	6	no	no	5 min	no	no	2.8 ± 0.9
LCM (n = 22)
F-control	11	10 min	2 h	no	1 min	no	7.6 ± 0.8
F	11	10 min	2 h	no	1 min	yes	5.8 ± 1.4

ISSN: 1471-2164