Figure 3

Heat map of hierarchical clustering of 249 cuticular protein genes in 16 developmental stages. Clustering was done using Cluster 3 software. The X-fold values of signal intensities divided by 800 were used in clustering. The colors in the map display the relative values of all tiles within the given 16 developmental stages. Green indicates the lowest expression, black indicates the intermediate expression, and red indicates the highest expression. The numerical values give the actual values on a log 2 scale, which were associated with each color. The color scale bar is shown at the top right corner of the figure. A: Genes clustered with respect to the 16 developmental stages (clustering type: hierarchical clustering, Distance metric: Pearson correlation). B: Genes grouped into five clusters on the basis of the expression similarity (clustering type: K-means clustering, Distance metric: Pearson correlation).