Figure 3

Real-time PCR validation of microarray results (A). Fold-change expression profiles of selected genes determined by real-time PCR. At each time point, bars indicate mean ± SEM (n = 5). (B) Correlation plot of fold-change values (control vs stressed fish) for 10 selected genes (at 4 sampling times) analyzed by microarray (X-axis) and PCR methodologies (Y-axis).