Identification of Gcn5 dependent KCl stress response genes in S. cerevisiae. (A) The Gcn5 dependency of global gene expression increases during KCl induced stress. Graph shows the frequency distribution (y-axis, arbitrary units) of the Gcn5 dependency of genes (x-axis) in the absence (black curve) and presence (red curve) of KCl. The Gcn5 dependency for each gene is expressed as the ratio between the mean transcript level in the gcn5Δ strain in relation to the mean transcript level in the wild type strain. (B) Diagram illustrating how Gcn5 dependent KCl response genes were defined. The selected genes are differentially regulated in wild type cells during KCl stress conditions in a fashion that requires Gcn5 when gcn5Δ is compared to wild type during KCl adaptation. Selection criteria are described in the Methods section. The number for of genes in each group is indicated. The heat map shows the fold change values for these genes in the two data sets used to identify them, +KCl/-KCl (wt) and gcn5Δ/wild type (gcn5Δ).