The ancient mammalian KRAB zinc finger gene cluster on human chromosome 8q24.3 illustrates principles of C2H2 zinc finger evolution associated with unique expression profiles in human tissues

Table 5 Frequent individual elements from TFBS modules in proximal promoter regions of the 8q24.3 ZNF genes ^a

TFBS family^b	Individual TFBS^b	Factor^c	ZNF7	ZNF16	ZNF34	ZNF250	ZNF251	ZNF252	ZNF517	Total	Promoter count^d
AP2F	AP2.02	AP-2α	2	0	0	1	1	1	2	7	5
EGRF	EGR1.02	EGR1	3	0	1	2	2	1	1	10	6
ETSF	CETS1 P54.01	c-ETS1 (p54)	1	0	0	0	0	1	1	3	3
	ELK1.02	ELK1	1	4	0	0	3	1	0	9	4
	SPI1_ PU1.02	SPI1	0	0	1	2	2	3	1	9	5
IKRS	IK2.01	IKZF1	1	0	0	0	1	1	1	4	4
SP1F	SP1.01	SP1	3	1	1	4	3	3	3	18	7
	SP1.03	SP1	2	0	0	3	3	3	1	12	5
		Total	13	5	3	12	15	14	10	72

^a Only TFBS module families that occur in promoter regions of at least three 8q24.3 ZNF genes served as input for counting. Listed are only TFBS that were found in at least three different promoter regions. The position in a module was not considered. It does not exlude the presence of additional binding sites within the promoter sequences.
^b TFBS family and individual TFBS matrices based on Genomatix Matrix Library 8.1. Derived from the results using Genomatix ModelInspector. Detailed listing and sequences in Additional file 14
^c Transcription factor recognizing the respective TFBS
^d Number of 8q24.3 ZNF promoter regions with matches of a given TFBS

ISSN: 1471-2164