Major changes in gene coding regions in the CMS lines. (A) Structure of orf152b. The deletion of nucleotides in the N-terminal region shortened the ORF to a length of 92 amino acids in the CW-CMS genome. (B) Structures of orf288. Nucleotide extension at the N-terminal region seen in the LD-CMS genome caused the generation of a novel ORF predicted to encode a 310-amino-acid polypeptide (L-orf310). The chimeric structure of partial orf288 for N-terminal peptides and sequences of unknown origin generated the CW-orf307 gene in the CW-CMS genome. (C) The 6,881-bp alien sequence insertion in the downstream region of atp6 in the LD-CMS genome.