Table 2 Quantitative PCR validation of microarray analysis
From: Testes and brain gene expression in precocious male and adult maturing Atlantic salmon (Salmo salar)
Gene | Microarray | qPCR | qPCR |
|---|---|---|---|
| Â | Testes Expression (Fold Change) | ||
| Â | P relative to NP | P relative to NP | November relative to Jul |
AMH | -7.2 | -58.9 | -231.6 |
ApoE | +4.8 | +2.5 | +1.2 |
EF1α | -1.8 | -5.3 | -3.7 |
β-Glob | -2.9 | -4.9 | +3.7 |
Cat B | +3.2 | -2.3 | 1.0 |
β2M | -1.7 | -3.4 | -2.1 |
LPL | +2.7 | +3.7 | +2.9 |
GNBP | -2.6 | -4.3 | -2.6 |
Col 1A | -4.0 | -16.7 | +5.8 |
| Â | Brain Expression | Â | |
ApoE | -1.3 | -1.4 | Â |
β-Glob | -1.5 | +1.3 |  |
IT-1 | n/a | -9.0 | Â |
IT-2 | -1.5 | -9.1 | Â |
MCH | +1.2 | +1.7 | Â |
02A07 | -1.3 | +1.1 | Â |
04H011 | +1.5 | +1.2 | Â |
22D02 | +1.3 | +3.0 | Â |