Figure 4
From: Acetylation of p65 at lysine 314 is important for late NF-κB-dependent gene expression
Acetylated p65 is recruited to the promoter region of regulated genes upon TNFα stimulation. (A) Promoters of Cfb, Mpa2l and Mmp10 have putative κB sites. Schematic representation of putative κB sites found in the indicated promoter regions. The distance in bp relative to the transcription start site (TSS) of every putative κB site is shown, as well as the sequence from the κB site chosen for analysis by ChIP. Chromatin immunoprecipitation analysis of p65 (B) or p65acetylated at K314 (C) from p65(+/+) MEFs kept unstimulated or treated with TNFα for 20 or 180 minutes. Chromatin from p65(-/-) MEFs stimulated with TNFα for 180 minutes was used as negative control. Recruitment to the indicated promoters was analyzed. Occupancy to IP-10 and Glucagon promoters was assessed as positive and negative control, respectively. Samples were normalized to input chromatin and expressed as % input. The result is representative of three independent experiments. Mean values ± SD of three independent runs are shown.