Differential transcript expression between the microfilariae of the filarial nematodes, Brugia malayi and B. pahangi

Table 5 Sequence of gene-specific primers used for real-time PCR analysis.

Pub locus	Forward primer	Reverse primer
Bm1_49585	GACCGAAACAAGAAAGGAAGCCGA	ACTTTCATCATCATTATGTCGTCGCG
Bm1_11050	GATGAAACGTTTCATGAGATG	GATAATCCTCCAAAACTACCG
Bm1_02070	AATAATTGGATTTCGAGTGAGAC	TTCATGCATAATTACCAGTTTCG
Bm1_28420	AAGGCTTCGATATGTTTCCATC	CTAACCACTAGTACATATTGTCT
Bm1_42865	AAACATGCAGTTAATGCACGAG	TGCTTGCAATTCATTCCTCACT
Bm1_19100	GCCACAAGGTATGCAACCACA	GTGTCTGCGTGGGTGGTAAC
Bm1_25950^a	GCTAACTTCAATGCACTTAGTG	AAATAAGAAACAACGTTTGATACAG
Bm1_01300^a	CAATTTTCAGACTCAATCTACTG	AGGTAAGTATTTGTCAACGTTTG
Bm1_44655^a	GTACGAAAGCAAGGATTCTGCT	CAGAATACTTTGTAGGTATCATTC
Bm1_50670^a	GTATTAGCTCAAGCAATGGAAG	CTAACTACTATCATCACTATTATC
bm.02018^ab	AGAATCAAATGTCCAGGAAG	CTGTGAGAGGAAGATAGTC
BMX9555^b	TTGATGTAGATGCTTTAACAAGTGCTGCTC	AAGAAATTGTCGACAAAGTCCGCAAG
BMX10185^b	CCGCACAACAATCCTCACTTGCT	TGTGTTACTGCTATTAACATCCTCACTGCC

Each primer is written in the 5' to 3' direction.
^a Indicates the genes whose probe regions were amplified and sequenced using the primers shown.
^b Indicates the probes whose primers were used to determine a suitable endogenous control for real-time PCR analysis.

ISSN: 1471-2164