Transcriptomic profiling of bovine IVF embryos revealed candidate genes and pathways involved in early embryonic development

Table 2 Gene Set Enrichment Analysis (GSEA) and GO enrichment analysis results with FDR = 0.25

GSEA pathways	Sample size (n/m)¹	P value	GO categories	Count/Expected count	P value
Enriched in degenerative embryos²			Biological process
Nuclear receptors	23/40	<0.001	Cholesterol metabolic process	10/1.4	<0.001
Monoamine GPCRs	20/33	<0.001	Steroid biosynthetic process	8/1.5	<0.001
Cell communication	76/138	<0.001	Small GTPase mediated signal transduction	18/8.3	<0.001
GPCRs class A rhodopsin like	78/185	<0.001	Cellular component
Cytokine pathway	18/22	0.003	Endoplasmic reticulum membrane	18/8.4	0.002
Enriched in normal embryos³			Molecular function
Biosynthesis of steroids	19/24	<0.001	Transferase activity, transferring alkyl or aryl groups	6/1.4	0.002
Met pathway	28/36	0.003
N-glycan biosynthesis	34/42	0.005
Linoleic acid metabolism	17/31	0.011

¹ n = number of genes in the analyzed dataset; m = number of genes in the original gene set
² Enrichment in degenerative embryo means significantly more genes in this gene set showed higher expression in degenerative embryos
³ Enrichment in blastocysts means significantly more genes in this gene set showed higher expression in blastocysts

ISSN: 1471-2164