Figure 4

AKAP13 is required for efficient phosphorylation of 12E8 tau. Shown in A are results of siRNA screening for AKAP13 siRNA. 12E8 tau is shown in red and total tau is shown in green (see Experimental Methods). For B, H4 cells overexpressing four repeat tau (4R0N) were transfected with siRNA targeting the AKAP13 transcript. Silencing of AKAP13 was confirmed with anti-AKAP13 antibody (top panel). Percent control values represent the average of three independent siRNA transfections and westerns shown. In A and B, NS refers to the non-silencing control.