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Table 1 Summary of sequencing results of the 20 sugarcane BACs using 454 Flex and the 2 BACs using Sanger method.

From: Microcollinearity between autopolyploid sugarcane and diploid sorghum genomes

General BAC clone information BAC sequence assembly Sugarcane - sorghum sequence alignment
No. BAC ID Insert size (kb) Chra. GenBank ID No. of contigs Assembled BAC (bp) No. of contig >10 k No. of un-ambigious contigsb Total (kb) No. of ordered contigs Total length of ordered contigs (kb) Spanned in sugarcane (bp) Spanned in sorghum (bp)
1 SC0126G04 75 1 FJ348725 8 86,848 4 7 85 5 79 59,458 61,142
2 SC0043O10 120 1 FJ348717 12 140,528 6 7 119 5 118 92,727 86,118
3 SC0159C20 80 2 FJ348726 22 136,575 4 19 135 8 100 57,277 41,718
4 SC0109D21 165 2 FJ348722 15 323,526 5 9 131 3 93 64,904 59,816
5 SC0215O02 40 3 FJ348732 14 90,423 4 10 79 3 38 26,478 25,458
6 SC0100B23 150 3 FJ348719 21 175,925 4 14 147 7 118 70,340 72,844
7 SC0104N03 130 4 FJ348720 15 131,592 6 13 131 6 95 57,524 50,074
8 SC0175O07 125 4 FJ348729 42 99,784 0 37 92 0 0 \ \
9 SC0019D20 0 5   0 0 0 0 0 0 0 \ \
10 SC0110 M04 145 5 FJ348723 15 147,646 6 11 127 4 99 41,367 37,981
11 SC0040E22 40 6 FJ348716 21 59,035 1 14 47 3 37 23,505 21,900
12 SC0204D12 90 6 FJ348731 5 96,221 3 4 96 2 76 39,070 27,519
13 SC0118L15 60 7 FJ348724 12 101,744 1 8 100 4 83 76,967 81,213
14 SC0187J16 110 7 FJ348730 11 119,506 6 10 119 4 55 31,930 27,526
15 SC0165P20 100 8 FJ348727 12 94,102 1 8 87 1 44 32,797 87,512
16 SC0150 M18 65 8 FJ348733 6 130,463 2 5 129 2 128 113,899 315,724
17 SC0108J08 60 9 FJ348721 12 113,932 3 8 104 5 98 79,663 69,498
18 SC0079A20 160 1 FJ348718 14 199,955 6 7 89 4 77 61,829 65,536
19 SC0172L01 80 10 FJ348728 19 52,373 0 18 50 0 0 \ \
20 SC0043H01 60 10 FJ348715 11 309,732 6 9 122 6 113 56,110 58,070
Total   1855    287 2,609,910 74 218 1989 72 1451 985,845 1,189,649
1 SC0118L15* 60 7 GU207345 6 62,255 3 6 62 6 62 61,625 91,428
2 SC0172L01* 80 10 GU207346 3 82,136 2 3 82 3 82 \ \
  1. *: Sanger sequence; a: corresponding sorghum chromosome; \: not available; a: chromosome number of sorghum based on the overgo probes' location.
  2. b: All 287 contigs were BLAST against each other, and those contigs with matching sequences to other contigs were removed from comparative analysis with sorghum genome