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Table 1 Sequence validation of putative SFPs

From: High-throughput marker discovery in melon using a self-designed oligo microarray

SFP per UniGene No. of genes FP TP PPV* Proportion of genes by SFP on array Proportion of genes by SFP number in sequence validation
1 118 21 97 0.82 0.68 0.55
2 62 17 60 0.78 0.19 0.29
3 21 9 20 0.69 0.07 0.10
>3 12 8 13 0.62 0.06 0.06
Total 213 55 190 *0.79 1 1
  1. Summary of bioinformatics analysis of 245 SFPs sequenced from the two different DNA pools of PI and 'Dulce'. The sequence data were compared to the database data. TP-True positive. FP-False positive. PPV-Positive predictive value.
  2. *Total PPV was recalculated by taking into account the actual representation of the different SFPs per UniGene group in the population. PPV = TP/[TP+FP] for each number of SFPs per gene category. The calculations were performed to adjust the validated SFP results with the proportions discovered by bioinformatics analysis.