From: Amplified fragment length homoplasy: in silico analysis for model and non-model species
Explanatory variables | |||||||
---|---|---|---|---|---|---|---|
Upstream parameters | AFLP parameters | AFLP diagnostics | |||||
Species | No. of sba | CG cont | No. of peaks per profile | Peak length | Homoplasy per peak | ||
Response variables | Distribution | (Df = 2) | (Df = 1) | (Df = 2) | (Df = 1) | (Df = 1) | (Df = 1) |
In silico analyses | |||||||
Peak length distributionb | Quasibinomial | F1,1349 = 2.09e-12, P = 1 | F1,1349 = 967.20, P < 0.001 | ||||
No. of peaks per profile | Negative binomial | χ2 = 1574.87, P < 0.001 | χ2 = 1869.47, P < 0.001 | χ2 = 275.24, P < 0.001 | |||
Homoplasy rate H | Quasibinomial | F2,278 = 0.98, P = 0.38 | F2,278 = 0.72, P = 0.49 | F1,278 = 576.26, P < 0.001 | |||
No. of CFc | Negative binomial | χ2 = 19.90, P < 0.001 | χ2 = 2.99, P = 0.22 | χ2 = 25.32, P < 0.001 | χ2 = 37.92, P < 0.001 | ||
No. of CFc in peaks with more than 10 fragments | Negative binomial | χ2 = 6.96, P = 0.03 | χ2 = 0.90, P = 0.64 | χ2 = 0.87, P = 0.35 | χ2 = 2.74, P = 0.10 | ||
Empirical analyses | |||||||
Fluorescence intensity | Negative binomial | χ2 = 22.33, P < 0.001 | χ2 = 2.39, P = 0.12 |