Figure 2

CDR3 nucleotide sequences retrieved from the cDNA clones. Sequences are shown from the codon after the cys-94 of the TRBV gene to the codon before the phe-104 of the TRBJ gene and grouped on the basis of the TRBD1 (a), TRBD3 (b), TRBD2 (c) or no TRBD usage (d). Nucleotides that are conserved in the 3' end of the V portion are considered of TRBV genomic origin and indicated in bold upper cases. Residues belonging to the different TRBJ genes, on the right, are indicated also in bold upper case at the 3' end of each sequence. The germline sequences of TRBD1, TRBD3 and TRBD2 gene are indicated at the top of each figure. The sequences considered to present recognizable TRBD genes (see text) are indicated in lower cases and nucleotide substitutions or insertions are underlined. Nucleotides that cannot be attributed to any coding elements (N-nucleotides) are indicated in capital letters on the left and on the right sides of the TRBD regions. Numbers in the right column indicate the level of 5'- TRBJ nucleotide trimming.