Figure 2

Enhancer activity of the conserved sequences in the 5'-flanking region of the Pax8 gene. A) All the CNS were subcloned upstream the minimal promoter E1b into the E1b-pGL3basic vector and transiently transfected in the rat thyroid cell line PC Cl3 (note: the CNS are not numbered in consecutive order). At 48 h post-transfection, transcriptional activity was determined as the firefly over renilla luciferase activity. Data are expressed as fold induction over the transcription obtained with E1b-pGL3basic, whose value was set at 1.0. Data are means from 3-4 independent experiments, each performed in duplicate; B) CNS87 was subcloned into the TK-pGL3basic vector (CNS87-TK) and its activity was compared with that of the CNS87-E1b vector in two different cell types. At 48 h post-transfection, transcriptional activity was determined as the firefly over renilla luciferase activity. Data are expressed as fold induction over the transcription obtained with the E1b-pGL3basic or the TK-pGL3basic vectors. Data are means from 3-4 independent experiments, each performed in duplicate.