Figure 4

TTF-1 binds to the FT-1 and FT-6 sequences. A) Results from competition assays of the FT-1 and FT-6 retarded bands with increasing excess of the indicated cold competitors; B) Protein extract of PC Cl3 and HeLa cells were incubated, alone or together with the antibody against TTF-1 or tubulin (as control), in a binding assay with probes FT-1, FT-6 and oligo C. The PC Cl3 specific supershift observed with the antibody against TTF-1 is clearly visible; C) ChIP experiment performed on PC Cl3 cells showing the binding in vivo of TTF-1 to the CNS87 element.