Transcripts encoding the highest expressed Bgh Y/F/WxC-effector candidates accumulate predominantly in haustoria. (A) Semi-quantitative RT-PCR on total RNA isolated from Bgh spores and hyphae collected from the leaf surface 7 days post inoculation (dpi) and from barley epidermal strips containing Bgh haustoria (surface fungal material removed). (B-E) qPCR on RNA form time-course of barley whole leaf/Bgh material. (B) Transcript accumulation of Bghβ-tubulin relative to the barley HvGAPDH transcript. (C-E) Transcript accumulation of Y/F/WxC-effector candidate transcripts relative to 0 dpi. Bghβ-tubulin used as internal standard.