Figure 1

Prostatosphere formation in LNCaP cell line. A. Morphology of LNCaP subpopulations plated in SCM only (-) or in SCM-1%KO (1%KO). Top: 2400 dpi scans. Bottom: 20 × lens phase contrast images.

B. Percentage (%) of sphere forming cells during 6 serial passages or generations (G 1-6).

C. Fluorescent microscopy images of Cell-tracing experiments with 20 × objective.