Figure 4

Expression of CSC markers in PSs. Heatmaps of the mRNA levels of literature-defined CSC markers in LNCaP and PCSCs. Each heatmap compares spheres growing in SCM-1%KO (spheres) and adherent cultures growing in commercial medium supplemented with 10% FBS (parental). Left panel shows three biological replicates of LNCaP cell line. Right panel shows the three individual primary cell cultures from PCSC-1 (1), PCSC-2 (2) and PCSC-3 (3) as indicated, and the result is the average of the two replicates for each cell line. Only genes that show a significant change (p > 0.05) are included in the heatmap.