Generation of a BAC-based physical map of the melon genome

BMC Genomics

Table 3 Summary of the C. melo anchored genetic map.

													Markers
Linkage Group:	I	II	III	IV	V	VI	VII	VIII	IX	X	XI	XII	mapping	TOTAL
													two LG
RFLP
Total Analyzed	8	14	10	10	7	10	6	11	8	8	10	5	10	117
Non-Anchored ^a	1	2	2	3	1	1	1	2	2	0	2	0			17
Anchored to BACs	7	12	8	7	6	9	5	9	6	8	8	5	10			100
Contigs	7	11	7	6	6	8	5	7	5	7	7	5	10				91
Singletons	0	1	1	1	0	1	0	2	1	1	1	0	0					9
Number of positive BAC clones	45	68	38	28	47	52	32	43	37	33	37	22	56						538
Anchored to Physical Map (FPC):
To Contigs	6	10	7	7	6	9	5	7	5	5	7	4	10							88
Only to Singletons	1	1	0	0	0	0	0	1	0	2	1	1	0								7
SNP
Total Analyzed	9	5	7	9	5	11	12	9	10	6	9	4		96
Non-Anchored ^a	1	0	0	1	3	4	2	2	1	1	3	2			20
Anchored to BACs	8	5	7	8	2	7	10	7	9	5	6	2				76
Contigs	7	5	5	6	1	3	9	6	7	5	5	2					61
Singletons	1	0	2	2	1	4	1	1	2	0	1	0						15
Number of positive BAC clones	34	23	24	29	5	19	46	24	33	23	30	14							304
Anchored to Physical Map (FPC):
To Contigs	8	5	6	7	1	4	10	6	9	4	6	2								68
To Singletons	0	0	0	0	1	1	0	1	0	1	0	0									4
SSR
Total Analyzed	0	0	0	0	0	2	0	0	0	0	0	0		2
Anchored to BACs						2										2
Contigs						1											1
Singletons						1												1
Number of positive BAC clones						3													3
Anchored to Physical Map (FPC):
To Contigs						1														1
To Singletons						1															1
													TOTAL:	215	37	178	153	25	845	157	12

^a Markers that failed to amplify when tested in the BAC library even though PCR amplification bands were detected when parental
genomic DNA was used as a positive control

ISSN: 1471-2164