Figure 4
From: Transcriptomic responses in mouse brain exposed to chronic excess of the neurotransmitter glutamate
Localization of PTK2B and PTK2BpY402 in dendrites of CA1 hippocampus neurons in Tg mice. Labelling with antibodies to PTK2B, PTK2BpY402, and MAP2A were performed exactly as in Figure 3. The labelled sections were imaged using confocal microscopy and are shown as super-imposed images of PTK2B and MAP2A (A) or PTK2BpY402 and MAP2A (B). Sections were obtained from 20 mo-old mice. Enlarged images of dendrite regions from the stratum radiatum enclosed by white squares in A and B are presented below the respective images. Note the relatively uniform distribution of PTK2B in dendrites of the CA1 region as compared to the large puncta of immunolabelling by anti-PTK2BpY402. Arrows point to the two types of labelling by anti-PTK2B and anti-PTK2BpY402 antibodies along the path of a dendrite in CA1 stratum radiatum. Bar: 10 μm.