Figure 5

Correlation of GeneChip data with real-time quantitative PCR or immunoreactivity data of nine important genes with significantly altered expression in the Glud1 mice. To validate the GeneChip data, confirmatory data were collected by both real-time quantitative PCR and Western blot analyses. Three replicate measurements were performed on each gene (or protein) and the mean value is shown here. Real-time quantitative PCR was used for the measurements of Arhgap12, Arhgef2, Arpc2 and Epha4, while Western blots were used for the products of Dlg4, Gria1, Grin2a, Grin2b and Ptk2b. The Pearson correlation coefficient (r) between the GeneChip and confirmatory data, and the estimated p value, are shown at the bottom. The best-fit linear regression line (solid line) and the estimated 95% confidence limits (dashed lines) are also shown.