Skip to main content

Table 3 Changes in expression of DSC1, DSG1 and DHRS7C levels between polled, horned and scurs evaluated by q-PCR.

From: Transcription profiling provides insights into gene pathways involved in horn and scurs development in cattle

Contrasts of interest Gene ΔΔCt* s.e. p-value q-PCR Fold change# Microarray fold change#
PvH DSC1 -3.48 1.2 0.0075 11.2 8.76
PvH DSG1 -2.95 1.4 0.0402 7.7 7.57
PvH DHRS7C -7.36 0.6 <0.0001 164 12.6
PvS DSC1 0.99 1 0.3213 -1.98 -0.927
PvS DSG1 0.81 0.9 0.359 -1.87 1.67
PvS DHRS7C -5.22 1.1 <0.0001 37.3 3.76
HvS DSC1 4.47 1.2 0.0004 -22.2 -9.5
HvS DSG1 3.75 1.2 0.0039 -13.5 -4.56
HvS DHRS7C 2.13 1 0.0459 -4.38 -3.35
  1. *sign of the difference is based on the reference gene corrected 1st phenotype Ct minus the reference gene corrected 2nd phenotype Ct in each contrast for respective target genes.
  2. # fold change is relative to the first phenotype e.g. a change of 11.2 in PvH would indicate that the gene showed 11.2 fold higher expression in polled versus horned tissues