Figure 1

Phylogenetic tree of CCEs. MEGA 4.0 [40] was used to construct the phylogenetic tree using the Minimum Evolution method. Asterisks in the cladogram indicate bootstrap values greater than 50%. The nomenclatures of clades or groups are according to Teese et al [23]. Subclades of CCEs showing 1:1 orthologous relationship are marked red. The CCEs of B. mori are colored light blue, H. armigera blue, S. littoralis red and E. postvittana purple. The tissues in which each CCE is expressed are shown to the right of CCE name. For B. mori CCEs, the number of EST clones identified by the database analysis is shown in parenthesis. Species name abbreviations: Ha, Helicoverpa armigera; Hv, Heliothis virescens; Bm, Bombyx mori; Ep, Epiphyas postvittana; Sl, Spodoptera littoralis; Snon, Sesamia nonagrioides; Apol, Antheraea polyphemus; Mbra, Mamestra brassicae; Ms, Manduca sexta; Cf, Choristoneura fumiferana. Abbreviations for tissues: mg, midgut; sg, silk gland; ov, ovary; he, hemocyte; fb, fat body; ep, epidermis; ant, antenna; phe, pheromone gland; br, brain; wg, wing; tho, thorax; abd, abdomen; ca, corpora allata; mxg, maxillary galea; prob, proboscis; pr, prothoracic gland; ce, compound eye; te, testis; Vg, Verson's gland; Mt, Malpighian tubules.