Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and Bos taurus cattle

Table 4 Microarray evaluation by qRT- PCR using fourteen highly expressed genes from different tick stages to confirm microarray differential expression (TC5872 and TC9228 are utilized in 2 comparisons).

ID	Description	Comparison	Microarry fold change	qRT-PCR fold change^a
CK173243	GGY domain protein	BFL × L	4.522	0.161
TC5847	Unknown	BFL × L	2.493	6.238^b
TC13140	ATSP homologue	BFL × L	1.856	0.304
CK174565	GGY domain protein	BFL × L	3.861	0.512
TC5872	GGY domain protein	BFL × L	2.609	0.791
TC9228	Lipocalin-like protein	BFL × L	2.876	3.888^b
TC6326	GGY domain protein	HFL × L	1.732	2.069^b
CK184446	Serpin-1	HFL × L	2.452	0.176
TC5872	GGY domain protein	HFL × L	2.073	0.6323
TC9228	Lipocalin-like protein	BFL × HFL	1.27	3.888^b
TC9672	Histamine binding protein 22.8kDa	BFL × HFL	1.249	1.459^b
TC5931	Serpin-2 precursor	BFL × HFL	1.294	1.124^b
TC9363	Histamine binding protein-1	B × H	6.713	9.955^b
TC12051	Immunoglobulin G binding protein B	B × H	4.446	8.748^b
TC9814	Unknown protein	B × H	4.124	4.715^b
TC8147	Putative salivary protein	B × H	3.016	0.484

^aqRT-PCR was conducted with a third cDNA preparation (sample 3) of the different tick stages.
^bThese qRT-PCR assays demonstrated a high correlation at 0.88 with the microarray data (linear regression with R squared 0.78)

ISSN: 1471-2164