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Table 4 Microarray evaluation by qRT- PCR using fourteen highly expressed genes from different tick stages to confirm microarray differential expression (TC5872 and TC9228 are utilized in 2 comparisons).

From: Comparative microarray analysis of Rhipicephalus (Boophilus) microplus expression profiles of larvae pre-attachment and feeding adult female stages on Bos indicus and Bos taurus cattle

ID Description Comparison Microarry fold change qRT-PCR fold changea
CK173243 GGY domain protein BFL × L 4.522 0.161
TC5847 Unknown BFL × L 2.493 6.238b
TC13140 ATSP homologue BFL × L 1.856 0.304
CK174565 GGY domain protein BFL × L 3.861 0.512
TC5872 GGY domain protein BFL × L 2.609 0.791
TC9228 Lipocalin-like protein BFL × L 2.876 3.888b
TC6326 GGY domain protein HFL × L 1.732 2.069b
CK184446 Serpin-1 HFL × L 2.452 0.176
TC5872 GGY domain protein HFL × L 2.073 0.6323
TC9228 Lipocalin-like protein BFL × HFL 1.27 3.888b
TC9672 Histamine binding protein 22.8kDa BFL × HFL 1.249 1.459b
TC5931 Serpin-2 precursor BFL × HFL 1.294 1.124b
TC9363 Histamine binding protein-1 B × H 6.713 9.955b
TC12051 Immunoglobulin G binding protein B B × H 4.446 8.748b
TC9814 Unknown protein B × H 4.124 4.715b
TC8147 Putative salivary protein B × H 3.016 0.484
  1. aqRT-PCR was conducted with a third cDNA preparation (sample 3) of the different tick stages.
  2. bThese qRT-PCR assays demonstrated a high correlation at 0.88 with the microarray data (linear regression with R squared 0.78)