Table 1 Sampling localities, tissue sources, and PCR primer sequences for the six vermetid species examined in this study
Species | FMNH voucher # GenBank accession # | Locality | Tissue | Primer Set A (5'-3') rrnL-F/cox1-R | Fragment Size (bp) | Primer Set B (5'-3') rrnL-R/cox1-F | Fragment Size (bp) |
|---|---|---|---|---|---|---|---|
Dendropoma maximum (Sowerby1825) | FMNH 318221 HM174253 | Gulf of Aqaba, Jordan | Foot tissue; buccal mass | CGAATTGAAAGGGGGGCTTGTGACCTCGATGTTG TTTCGATCCGTTAAAAGCATAGTGATAGCTCC | 6877 | ACGCTACCTTCGCACGGTCAAAGTACCGCGGC TTGTTATGCCAATAATGATTGGTGGTTTCGG | 8809 |
Dendropoma gregarium Hadfield & Kay 1972 | FMNH 318222 HM174252 | HI, USA | Foot tissue; buccal mass | CAAATCGAAAAAAGGGTTTGCGACCTCGATGTTG TTACGGTCAGTTAAGAGTATGGTAATAGCACC | 6490 | ATGCTACCTTTGCACGGTCAGGGTACCGCGGC TGGTAATACCCATGATAATTGGAGGTTTTGG | 9254 |
Eualetes tulipa (Chenu1843) | FMNH 318223 HM174254 | Peanut Island, Palm Beach Co., FL, USA | Buccal mass | CATATCGAAAGAATAGTTTGCGACCTCGATGTTG TTTCGGTCCGTCAACAATATTGTAATTGCCCC | 6880 | TTCAACGAGAGCGACGGGCGATATGTACAC (rrnS-R) TGGTAATGCCTATAATGATTGGGGGGTTCGG | 7472 |
Thylacodes squamigerus1 (Carpenter, 1857) | FMNH 318997 HM174255 | Corona del Mar, CA, USA | Foot tissue | CCCATCGAAAGAAGAGTTTGTGACCTCGATGTTG TTTCGGTCCGTCAACAGCATAGTAATAGCTCC | 6648 | ATGCTACCTTTGCACGGTCAGAGTACCGCGGC TGGTTATACCAATAATAATTGGTGGCTTCGG | 9034 |
Thylaeodus sp. | FMNH 318224 HM174256 | Kewalo Marine Laboratory, HI, USA | Head, foot & mantle margin | CATATTGAAAAAAAAGTTTGTGACCTCGATGTTG TTTCGATCAGTCAATAACATAGTAATTGCGCC | 4703 | ATGCTACCTTTGCACGGTCAGAGTACCGCGGC TAGTAATACCTATAATAATTGGTGGATTTGG | N/A |
Vermetus erectus Dall, 1888 | FMNH 318225 HM174257 | Pourtales Terrace, Florida Keys; FL, USA | Foot tissue; buccal mass | CTAATCGAAGAAAAGGCTTGTGACCTCGATGTTG TTTCGGTCAGTCAGTAGTATAGTAATAGCACC | 3107 | ATGCTACCTTAGCACGGTTAAAATACCGCGGC TTGTCATGCCTATAATAATTGGCGGATTTGG | N/A |