Figure 3

Up-regulation of BAT-selective genes and down-regulation of WAT-selective genes in the WAT of FSP27- deficient mice. (A & B) Relative expression levels of BAT-specific genes (COX8b, LSDP5, ELOVL3 and UCP1) in the WAT (A) and BAT (B) of wild-type (+/+) and FSP27- deficient (FSP27^-/-) mice. (C) Expression levels of BAT-specific genes in the WAT of leptin-deficient (ob/ob) and leptin/FSP27 double-deficient (ob/ob/FSP27^-/-) mice. (D & E) Relative mRNA levels of WAT-selective genes (MEST, RETNLα, leptin, adiponectin and TNFα ) in the WAT of wild-type (+/+) and FSP27-null (FSP27^-/-) mice, leptin-deficient (ob/ob) and leptin/FSP27 double-deficient (ob/ob/FSP27^-/-) mice (D) and in the BAT of wild-type (+/+) and FSP27-null (FSP27^-/-) mice. (E). The bars represent the mean ± S.E.M. ***P < 0.001, **P < 0.01 and *P < 0.05. The expression levels of each gene were assessed by semi-quantitative real-time PCR analysis. Three-month-old male mice were used in the analyses depicted in this and following figures unless otherwise noted.