Table 2 Bacterial strains and plasmids used in this study
Strains and plasmids | Characteristics | Source/reference |
|---|---|---|
Strains | Â | Â |
S. Enteritidis PT4 | Wild-type clinical isolate | 34α |
PT4 (pKOBEGA) | PT4 containing helper plasmid pKOBEGA, AmpR | This study |
PT4ΔssaN | PT4 ΔssaN::Km, in-frame deletion, KmR | This study |
PT4ΔssaR | PT4 ΔssaR::Km, in-frame deletion, KmR | This study |
PT4ΔsspH2 | PT4 ΔsspH2::Km, in-frame deletion, KmR | This study |
E. coli MC4100 | Used as template for amplication of kanamycin resistance gene | Gift from J. M. Ghigo |
Plasmid pKOBEGA | Vector for recombination experiments, AmpR | Gift from J. M.Ghigo |