Identification of conserved regions of RelE. Plot showing the percentage divergence from the pTRACA22 RelE sequence of homologous RelE sequences. Bars represent the percentage divergence in identical amino acids at each position of the pTRACA22 RelE sequence, based on alignments of RelE sequences from human gut metagenomes, plasmids and bacterial chromosomes. The pTRACA22 sequence is presented with the N terminus at the origin of the plot, and the N terminal M start codon omitted. Symbols indicate conserved residues (stars) and those at which between 90 and 100% of represented sequences exhibit a conserved substitution (circles), as indicated by the associated key.