Figure 1
From: Gill transcriptome response to changes in environmental calcium in the green spotted puffer fish
Blood plasma total calcium and ecac gill mRNA expression upon exposure to different water [Ca2+]. Each bar is the mean ± S.E.M of (A) the plasma total calcium levels (mM) and (B) the relative expression of the epithelial calcium channel mRNA in the gills (ecac expression quantified by qPCR and normalized to the reference gene rps18) of T. nigroviridis individuals (n = 5-6/group) after 2 or 12 h transfer to 10 ppt artificial water containing 2.9 mM Ca2+ (control water, black bars), 10 mM Ca2+ (HighCa water, light grey bars) or 0.01 mM Ca2+ (LowCa water, dark grey bars). Different letters indicate statistically significant differences (p < 0.05) between groups, evaluated by two-way ANOVA.