Gill transcriptome response to changes in environmental calcium in the green spotted puffer fish

BMC Genomics

Table 1 Summary of tag extraction from the T. nigroviridis gill SuperSAGE libraries

Library	C2 h	LowCa 2 h	C12 h	HighCa 12 h	LowCa 12 h	Total	Average	% of total (average)⁴
Tag extraction from sequencing data
Total extracted tags ¹	65,378	87,388	67,996	60,142	63,471	344,375	68,875
# unitags ²	24,536	31,907	27,403	24,427	24,886		26,632
% unitags	37.5	36.5	40.3	40.6	39.2		38.7
Distribution of Unitags by abundance classes ³
1	16,630	22,087	19,049	17,047	17,218	92,031	18,406	69.1
2 to 4	5,516	6,891	5,886	5,304	5,359	28,956	5,791	21.7
5 to 9	1,503	1,830	1,618	1,330	1,487	7,768	1,554	5.8
10 to 100	852	1,043	819	717	793	4,224	845	3.2
>100	35	56	31	29	29	180	36	0.1

¹Total number of tags extracted from each library after quality selection. ² Unique tags (or Unitags, corresponding to unique transcripts) obtained for each library. ³ Number of Unitags in each abundance class, based on their tag count in each library; ⁴ Percentage of Unitags in each abundance category relative to the total number of Unitags per library (on average).
SuperSAGE libraries prepared from gills in control water for 2 or 12 h (C2 h and C12 h); low calcium water for 2 or 12 h (LowCa2 h and LowCa12 h) or high calcium water for 12 h (HighCa12 h).

ISSN: 1471-2164