Figure 2

meiotic phenotypes of sum1Δ cells. A: The growth resulting from drops of germinating spores of wild-type and sum1Δ strains were compared at two temperatures. sum1Δ spores are more heat sensitive than the spores of the wild-type strain. B: Drops of germinating spores of wild-type and sum1Δ strains which were incubated with Zymolase for indicated times. After treatment with Zymolase, sum1Δ spores do not germinate, while wild-type cells still do. C: percentage of asci with 3-4 spores and asci with 1-2 spores in different acetate concentrations in the sporulation medium. The results are compared between wild-type and sum1Δ meiosis. sum1Δ cells do not react as well as wild-type cells to the reduction in acetate concentration, always keeping a lower amount of asci with 1-2 spores.