Figure 3

Immunofluorescence results of Hif1α expression in untreated controls (A'), BMS- (B') and BMS+FA-treated (C') embryos at 8.5dpc. Transverse sections of untreated controls (A), BMS-treated (B) and BMS+FA-treated (C) embryos at 8.5 dpc were counterstained with Hoechst to show nuclei (blue). Positive Hif1α staining is evident in the myocardium of untreated controls (A') and BMS+FA-treated embryos (C'). Bc = bulbus cordis; Da = dorsal aorta; Ec = endocardium or endocardial tissue; Fd = foregut diverticulum; Lhsv = wall of the left horn of sinus venosus; Mc = myocardium; Nt = neural tube; Pv = primitive ventricle