Figure 3

Alternative Splicing Events in genes known to have AS. The grey bars along the top of each diagram represent the RefSeq gene annotation. Traces in the upper region of each figure show average intensities of probesets representing exon regions for tumors (blue) and Cells (red). (a) Shows an example where the alternative splicing index for the GEM gene has a very low p value indicating alternative splicing events, however the fold change values for the entire transcript are ~5 fold suggesting that this is differential expression (b) The tumor tissue is expressing the full length isoform B of the OSBPL1A gene, while the cells are expressing the truncated version, isoform A, which has an alternative 5' start. (c) shows a classic example of a cassette exon, where the tumors are expressing isoform 1 of the PRKCSH gene and = the colon epithelial cells are representing isoform 2. (d) demonstrates another example of a cassette exon in the PYCARD gene. The third exon is not expressed in the tumor (α-isoform) but is expressed in the cells (β-isoform). (e) shows an example 3' alternative splicing in the PHF12 gene and (f) displays an example of a 5'splicing event in the DALRD3 gene.