Figure 1

Sampling conditions and rRNA fragmentation. (A) Three samples were taken during free-living growth based on the OD of the culture; early exponential phase (OD₆₀₀ = 0.3), late exponential phase (OD₆₀₀ = 0.7) and stationary phase (6 hours after reaching the maximum OD₆₀₀). (B) An example of high quality total RNA, illustrating the fragmentation 23S rRNA: (a) small RNA peak including 5S rRNA and the ncRNAs (b) 23S fragment of ~135 bp (c) two 23S fragments of ~1300 bp (d) 16S rRNA (e) intact 23S rRNA.