Conservation of R. etli ncRNAs in α-proteobacteria. Similarity analysis was performed using BLASTN (NCBI). Black squares indicate E-values ≤ 10-5, grey squares represent E-values ≤ 10-3. The percentage of conservation for each species is shown at the bottom of the figure. Only ncRNAs conserved beyond A. tumefaciens str. C58, R. etli CIAT652, R. leguminosarum bv. viciae 3841, S. meliloti 1021 and Mesorhizobium loti MAFF303099 are shown. Conservation analysis results of all identified ncRNAs are included as supplementary data, see Additional file 5, Table S3.