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Table 2 Media and treatment for growing G. clavigera mycelium for cDNA library construction

From: Gene discovery for the bark beetle-vectored fungal tree pathogen Grosmannia clavigera

Media composition
wood 10 g/plate lodgepole pine sawdust
starch 0.17% YNB, 0.1% PHP, 0.3% asparagine, 1% starch
organic nitrogen 0.17% YNB, 0.1% PHP, 0.3% asparagine, 1% maltose
inorganic nitrogen 0.17% YNB, 0.1% PHP, 0.3% NaNO3, 1% maltose
olive oil 0.17% YNB, 0.1% PHP, 0.3% asparagine, 1% (v/v) olive oil emulsified in 0.5% tergitol (olive oil and tergitol were mixed and autoclaved separately before being added to the media)
LPPE treatment Cultures were grown on organic nitrogen medium for 48 h and then sprayed with LPPE
  1. All media were solidified with 1.5% granulated agar. LPPE = lodgepole pine methanol extract, YNB = yeast nitrogen base, PHP = potassium hydrogen phthalate