Table 7 Primers used for quantitative RT-PCR analysis of unigene locations (ULs)
From: Gene discovery for the bark beetle-vectored fungal tree pathogen Grosmannia clavigera
Target UL | Primer name | 5'-3' sequence |
|---|---|---|
3230 | R1718-F1 | GTG TCC TCC ACC TTC CTC ACC |
R1718-R1 | CGT GAC TCC CTT GAC TTC TGG G | |
Gc_00052 | Gc_0052-F1 | GCT CTC TCT TTT GCC GGC GGA |
Gc_0052-R1 | GAG CCG GCC AGC GTT GAG TAA | |
Gc_00102 | Gc_00102-F1 | TCG GAC GGA CTG CAA ACG CG |
Gc_00102-R1 | CGA GCC CCA GAA AAG GAC GAC | |
3233 | R1719-F1 | CTC AGC AAC GGT CCA ACC TC |
R1719-R1 | GTG CTT CTT CCA CTT GCG GG | |
3234 | F1718-F3 | GAGCTGCTGACGCTCGATAA |
F1718-R3 | ACCTGACTGCTGTCGTCCAT |