Figure 2
From: Identification of the Rage-dependent gene regulatory network in a mouse model of skin inflammation
In silico promoter analysis of differentially expressed genes 24 hours after TPA treatment. (A) Annotated promoter sequences of 97 differentially expressed genes between wt and Rage-/- skin were screened for significant enrichment of transcription factor binding sites (TFBS). TFBS were grouped according to their enrichment within clusters of differentially expressed genes between wt and Rage-/- skin 24 hours after TPA treatment. (B) Protein expression of E2f1, E2f4, Rb, and Rbl1 was investigated by Western blot analysis with whole cell lysates of TPA- and control-treated back skin of wt and Rage-/- mice. Actin protein levels served as control for protein quality and quantity.